大孔吸附树脂纯化愈肾颗粒中总黄酮工艺

谢雪; 张宏达; 陈昱竹; 任国杰; 王晓玲; 许枬

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大孔吸附树脂纯化愈肾颗粒中总黄酮工艺

更新时间：2024-01-04

- 大孔吸附树脂纯化愈肾颗粒中总黄酮工艺
- Purification Technology of Total Flavonoids from Yushen Granules by Macroporous Resin
- 中国实验方剂学杂志 2011年17卷第20期页码：8-10
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- 作者简介：
- 基金信息：
  
  十一五重大新药创制项目(2009ZX09103-394)
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- 纸质出版日期：2011
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谢雪, 张宏达, 陈昱竹, 等. 大孔吸附树脂纯化愈肾颗粒中总黄酮工艺[J]. 中国实验方剂学杂志, 2011,17(20):8-10.

XIE Xue, ZHANG Hong-da, CHEN Yu-zhu, et al. Purification Technology of Total Flavonoids from Yushen Granules by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(20): 8-10.
谢雪, 张宏达, 陈昱竹, 等. 大孔吸附树脂纯化愈肾颗粒中总黄酮工艺[J]. 中国实验方剂学杂志, 2011,17(20):8-10. DOI：

XIE Xue, ZHANG Hong-da, CHEN Yu-zhu, et al. Purification Technology of Total Flavonoids from Yushen Granules by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(20): 8-10. DOI：

摘要

目的: 研究大孔吸附树脂法纯化愈肾颗粒总黄酮最佳工艺。方法: 以总黄酮的比吸附量、解析率、洗脱率等为指标

选用7种大孔吸附树脂对愈肾颗粒总黄酮进行纯化。结果: HPD100型大孔吸附树脂对愈肾颗粒的总黄酮具有较好的分离纯化能力。最佳工艺条件为2 BV的愈肾颗粒供试品溶液为上样量(含生药量为1 g·mL-1)

吸附速率为1 BV·h-1

分别用水2 BV、30%乙醇3 BV、50%乙醇4 BV洗脱

洗脱流速4 BV·h-1

合并30%乙醇和50%乙醇洗脱液

浓缩

即为纯化的总黄酮。结论: 纯化后总黄酮含量提高到76%以上

HPD100型大孔吸附树脂可较好的纯化愈肾颗粒总黄酮。

Abstract

Objective: To study on optimum purification technology of total flavonoids from Yushen granules by macroporous resin. Method: With adsorption capacity

desorption rate and elution rate as indexes

purified total flavonoids of Yushen granules by seven macroporous resin. Result: HPD100 macroporous resin showed good separation and purification capabilities of total flavonoids from Yushen granules.The optimum technology conditions were as follows:2 BV sample(dose of raw drug was 1 g·mL-1) of Yushen granules formula was passed through the column with flow rate of 1 BV·h-1

eluted by 2 BV water

3 BV 30% ethanol

4 BV 50% ethanol respectively

elution flow rate was 2 BV·h-1.Combined 30% and 50% ethanol elutes were concentrated to yield the purification of total flavonoids. Conclusion: The purity of total flavonoids was up to 76%. HPD100 could be well used in purifying total flavonoids from Yushen granule.

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