Objective: To investigate the effects of Huoxiang Zhengqi Liquid on expression of Zounlaoccludens-1(ZO-1) in ileum mucosa of rats with dampness retention syndrome(DRS). Method: Twenty four SD rats were randomly divided into 3 groups: control

DRS group and treatment groups (DRS + Huoxiang Zhengqi Liquid)

8 rats in each group.DRS was induced in all rats

except the control group

by modified enviromental and fatigued method for 6 days.After DRS model was formed

control and DRS groups were lavaged with normal saline(20 mL·kg-1). Rats in treatment groups were lavaged with Huoxiang Zhengqi Liquid(20 mL·kg-1) for 8 days.Then expression of ZO-1 on ileum mucosa was determined by Real-Time PCR. Result: Rats of model group exhibited typical DRS

with sign of hyperaemia

edema

hemorrhagic spots and sludged blood in the gastric mucosa was compared to control. The expression of ZO-1 on ileum mucosa significantly decreased in DRS group compared to that of control group(P<0.01).Conversely

exposure of DRS rats to Huoxiang Zhengqi Liquid resulted in increase in expression in ZO-1 in the ileum mucosa (P<0.01 versus DRS group). Conclusion: Huoxiang Zhengqi Liquid could have the protective effect on DRS-induced gastric mucosal injury

and increasing in expression of ZO-1 may play an important role.