大孔吸附树脂分离纯化芫花黄酮苷元工艺研究

夏林波; 李博; 邓仕任; 贾天柱; 英锡相

您当前的位置：

首页 >

文章列表页 >

大孔吸附树脂分离纯化芫花黄酮苷元工艺研究

更新时间：2024-01-04

- 大孔吸附树脂分离纯化芫花黄酮苷元工艺研究
- Studies on Separation and Purification of Flavonoid Aglycone from Flos Genkwa by Macroporous Adsorption Resin
- 中国实验方剂学杂志 2011年17卷第17期页码：13-16
- 作者机构：
- 作者简介：
- 基金信息：
  
  辽宁中医药大学药学人才基金 (yxrc0916)
- DOI：
  中图分类号：
- 纸质出版日期：2011
- 稿件说明：
移动端阅览
夏林波, 李博, 邓仕任, 等. 大孔吸附树脂分离纯化芫花黄酮苷元工艺研究[J]. 中国实验方剂学杂志, 2011,17(17):13-16.

XIA Lin-bo, LI Bo, DENG Shi-ren, et al. Studies on Separation and Purification of Flavonoid Aglycone from Flos Genkwa by Macroporous Adsorption Resin[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(17): 13-16.
夏林波, 李博, 邓仕任, 等. 大孔吸附树脂分离纯化芫花黄酮苷元工艺研究[J]. 中国实验方剂学杂志, 2011,17(17):13-16. DOI：

XIA Lin-bo, LI Bo, DENG Shi-ren, et al. Studies on Separation and Purification of Flavonoid Aglycone from Flos Genkwa by Macroporous Adsorption Resin[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(17): 13-16. DOI：

摘要

目的: 研究考察大孔吸附树脂用于芫花中2种主要黄酮苷元-芹菜素和芫花素的分离纯化效果。方法: 采用静态和动态吸附法

利用高效液相对芹菜素和芫花素进行定量分析

筛选最佳分离纯化工艺。结果: 最佳工艺条件为AB-8树脂

上样液的质量浓度0.1 g ·mL-1

吸附流速2 BV ·h-1

上样体积5 BV。再以9 BV 95%乙醇洗脱

洗脱流速2 BV ·h-1

体积9 BV。经树脂处理后2种黄酮苷元纯度均达到20%以上。结论: 大孔树脂对芫花中芹菜素和芫花素具有较好分离纯化效果

优选工艺操作简单、稳定可行。`

Abstract

Objective: To investigate the purification method of two main flavonoid aglycones (apigenin and genkwanin) from Flos Genkwa by macroporous resin. Method: HPLC was used to determine the content of apigenin and genkwanin

and the purification conditions were selected by static and dynamic adsorption. Result: AB-8 resin was selected because it gave the most effective of separation. The optimum process for the purification was as follows: the concentration of sample solution was 0.1 g ·mL-1

the dosage was 5 BV

adsorption flow rate was 2 BV ·h-1

and eluted with 95% ethanol by 9 BV

elution flow rate was 2 BV ·h-1. The purity of two flavonoid aglycones was more than 20% after processing. Conclusion: Apigenin and genkwanin were purified effectively with macroporous resin. The optimum process is simple

stable and feasible.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

多成分同时比较芫花及芫花叶化学组成

芹菜素通过PI3K/Akt和MAPK信号通路诱导人大肠癌CL187细胞凋亡

秋鼠曲草花部分有效成分提取工艺优选

UPLC-QTOF/MS分析芫花诱导人肝细胞L02损伤的毒性物质基础

芹菜素抗癌机制研究进展