Objective: To investigate the purification technology of total alkaloids from Rhizoma Coptidis by macroporous resin

and to provide feasible program for large-scale manufacture. Method: Evaluated the effect of purification of total alkaloids from Rhizoma Coptidis by D101

AB-8 and D151 resins respectively with the indicator of the content of berberine. Result: It has been found that D151 resin’s adsorption capacity of total alkaloids was the best in those three resins. The optimum process was as follows: the concentration of the sample solution was 0.15 g ·L-1 with 9 times the amount of resin volume

washed 2 times the amount of resin volume by distilled water and 5 times the amount of resin volume by 0.12 mol ·L-1 of HCl solution. Under those conditions

more than 60% extract was reduced with the proportion of total alkaloid in excess of 80%

and 70% of sample recover. Conclusion: D151 resin is suitable for industrial production for excellent drug loading and purification.