微波协助提取在中药材含量测定中的应用(2)——微波法与药典法测定白芷中欧前胡素含量比较

方婧; 付梅红; 杨洪军; 张贝贝; 王祝举; 杨岚; 张东

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微波协助提取在中药材含量测定中的应用(2)——微波法与药典法测定白芷中欧前胡素含量比较

更新时间：2024-01-04

- 微波协助提取在中药材含量测定中的应用(2)——微波法与药典法测定白芷中欧前胡素含量比较
- Determination of Microwave-assisted Extraction of Chinese Herbs (2) Comparison of Determination of Imperatorin from between Microwave-assisted Extraction and Chinese Pharmacopeia Method
- 中国实验方剂学杂志 2011年17卷第20期页码：89-91
- 作者机构：
- 作者简介：
- 基金信息：
  
  "重大新药创制"科技重大专项(2009ZX09308-003);国家科技支撑计划课题(2006BAI09B07-02)
- DOI：
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- 纸质出版日期：2011
- 稿件说明：
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方婧, 付梅红, 杨洪军, 等. 微波协助提取在中药材含量测定中的应用(2)——微波法与药典法测定白芷中欧前胡素含量比较[J]. 中国实验方剂学杂志, 2011,17(20):89-91.

FANG Jing, FU Mei-hong, YANG Hong-jun, et al. Determination of Microwave-assisted Extraction of Chinese Herbs (2) Comparison of Determination of Imperatorin from between Microwave-assisted Extraction and Chinese Pharmacopeia Method[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(20): 89-91.
方婧, 付梅红, 杨洪军, 等. 微波协助提取在中药材含量测定中的应用(2)——微波法与药典法测定白芷中欧前胡素含量比较[J]. 中国实验方剂学杂志, 2011,17(20):89-91. DOI：

FANG Jing, FU Mei-hong, YANG Hong-jun, et al. Determination of Microwave-assisted Extraction of Chinese Herbs (2) Comparison of Determination of Imperatorin from between Microwave-assisted Extraction and Chinese Pharmacopeia Method[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(20): 89-91. DOI：

摘要

目的: 比较微波协助提取法与药典法提取欧前胡素优势。方法: 采用Dikma Diamonsil C18色谱柱(4.6 mm×150 mm

5 μm)

流动相为甲醇-水(55 ∶45)

检测波长300 nm

柱温30 ℃

流速1 mL·min-1。结果: 微波提取时间10 min

提取温度80 ℃

提取溶剂乙醇。欧前胡素在0.01~0.2 μg呈良好线性关系(r=0.999 9)。平均回收率97.48%(n=6)。结论: 微波提取法较药典法提取欧前胡素更简便快速、结果准确

为一种符合环保绿色理念的含量测定方法

可用于检测白芷药材中有效成分欧前胡素含量。

Abstract

Objective: To establish a microwave-assisted extraction method for the extraction of imperatorin from Angelica dahurica(Fisch.ex Hoffm.) . Comparison of the determination of imperatorin from A. dahurica between microwave-assisted extraction and Chinese pharmacopeia method was carried out. Method: Dikma Diamonsil C18 column (4.6 mm×150 mm

5 μm) was used. The mobile phase consisted of methanol-water (55 ∶45). The temperature of column was at 30 ℃. The flow rate was 1.0 mL·min-1 and the detection wavelength was at 300 nm. Result: Microwave extractive time was 10 min

the temperature was at 80 ℃

the extraction solvent was ethanol. The calibration curve was linear in the range from 0.01 to 0.2 μg (r=0.999 9). The average recovery was 97.48% (n=6). Conclusion: The method is simple

rapid

as well as precise and reliable

and can be used for the determination of imperatorin from A. dahurica.

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