Objective: To observe the protect effects of chlorogenic acid on liver-injury of mice induced by Carbon Tetrachlotide (CCl4). Methods: Animals were devided into 6 groups:normal group

model group

solvent group

the chlorogenic acid high-dose group

middle-dose group

low-dose group. The dose of chlorogenic acid is ig 14

7

3.5 g·kg-1respectively. The mice models of acute chemical liver injury was induced by CCl4 . The indexes of the live and spleen

alanine transaminase(ALT) and aspartate transaminase(AST) activity in serum were examined. Superoxide dismutase(SOD)

glutathione peroxidase(GSH-Px)activity

MDA level in liver tissue were determined by immunological liver injury. Meanwhile liver pathological examination was observed by light microscope. Result: The chlorogenic acid (14

7

3.5mg·kg-1)could remarkablly resist the increase of ALT(40.01±7.14)

(51.19±9.45)

(62.66±9.01)U·L-1

AST (43.59±4.32)

(52.99±5.97)

(66.97±7.34)U·L-1

decreased the MDA level (21.68±10.28)

(28.54±6.84)

(35.51±9.87) nmol·g-1

and could improve SOD activity (112.98±8.41)

(98.36±11.18)

(81.76±9.88) U·g-1and GSH-Px level (196.19±10.56)

(169.77±13.61)

(141.57±15.11) U·g-1 (P<0.01 versus model group for above results). Conclusion: Chlorogenic acid has remarkable protective effects on liver –injury in mice.