Objective: To study the difference of the process parameters with the three kinds of Chinese herbs polysaccharides by hot water extraction

and to investigate the possibility of combination extraction by orthogonal experiments. Method: Optimize extraction process of three kinds of polysaccharide respectively by orthogonal test

compare yield and water-solubility of polysaccharide extracted with optimal hot water extraction

alkali extraction and sonic extraction. Result: Optimal extraction processes of Rhizoma Chuanxiong polysaccharide was: the temperature of extraction was at 90 ℃

the ratio of sample to solution was 1∶20

and extraction time was 1 h. Optimal extraction processes of Radix Paeoniae Rubra polysaccharide was the temperature of extraction at 80 ℃

he ratio of sample to solution 1∶20

and extraction time 4 h. Optimal xtraction processes of Cortex Phellodendri Chinensis polysaccharide was extraction temperature 90 ℃

the ratio of sample to solution 1∶10

and extraction time 4 h. At the same time compared with polysaccharides extracted by the other two methods

polysaccharide extracted by hot water extraction has higher yield and solubility. Conclusion: The hot water extraction is more suitable for combination extraction.