SHAO Jing, FAN Qin, GUO Mei, et al. Optimization of Extraction Conditions of Niubang Gancao Tang by Orthogonal Design[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(14): 46-48.
SHAO Jing, FAN Qin, GUO Mei, et al. Optimization of Extraction Conditions of Niubang Gancao Tang by Orthogonal Design[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(14): 46-48.DOI:
Objective: To optimize the water extraction conditions of Niubang Gancao Tang. Method : The L9 (3)4 orthogonal method was employed to study the best extracted technics through analyzing the content of arctiin by HPLC and the weight of ointment.The determination was carried out on a Hypersil-Keystone C18 column with the mobile phase of methanol and water (43 ∶57)
detection wavelength of 280 nm. Result : The best technics of extraction process is 10 times amount of water
decocted 3 times
2 hours each time. Validatvon test showed that 20.69% arctiin content
dry extract yield was 24.37%. Conclusion : Optimum conditions of water extraction of Niubang Gancao Tang are obtained and the optimized extraction technology is stable and efficient.