Objective: To study the HPLC fingerprint of hawthorn leaf from different producing areas

establish a method for the analysis of chlorogenic acid

glucosyl-vitexin

vitexin-2″-O-rhamnoside

hyperoside and quercetin. Method: The separation was carried out on an Agilent ZORBAX SB-C18 column(4.6 mm×250 mm

5 μm)with the mixture of acetonitrile and 0.5% formic acid as mobile phase in a gradient elution model(flow rate 1.0 mL·min-1

detection wavelength 350 nm

column temperature 30 ℃). 13 groups of hawthorn leaf were analyzed with the developed HPLC fingerprint and determination methods

the data calculation was performed with similarity evaluation system for chromatographic fingerprint of TCM(Version 2004A). Result: The linear range of chlorogenic acid

glucosyl-vitexin

vitexin-2″-O-rhamnoside

hyperoside and quercetin were 3.75-120 mg·L-1(r=0.999 6)

9.69-310 mg·L-1(r=0.999 8)

24.69-790 mg·L-1(r=0.999 9)

6.25-200 mg·L-1(r=0.999 9)

0.31-10 mg·L-1(r=0.999 9)

respectively. The average recovery rates of chlorogenic acid

glucosyl-vitexin

vitexin-2″-O-rhamnoside

hyperoside and quercetin were 99.2%(RSD 1.7%)

100.3%(RSD 1.8%)

99.0%(RSD 1.4%)

99.8%(RSD 2.1%)

100.5%(RSD 1.9%)

respectively. In the fingerprint

15 common peaks of hawthorn leaf were confirmed and 5 peaks were determined. Conclusion: This method provides a scientific basis for controlling the quality of hawthorn leaf.