Objective: Choose the best enzyme and craft conditions of hypericin from Hypericum perforatum. Method : The content of hypericin determined by HPLC was used as an index. The orthogonal experiments were used for the optimal craft conditions choosing two of the best enzymes in cellulase

pectinase

xylanase

glucanase

β-glucanase and enzyme (SPE-007A) to investigate the effect on their complex by solid-liquid ratio

enzyme dosage

pH

temperature and the extraction time. Result : The two best enzymes are cellulase and pectinase

the optimum extraction process is as follows pH 4.8

enzyme dosage cellulase-pectinase 2.0%-2.0%

temperature 50 ℃

extraction time 6 h. Conclusion : This method is efficient and stable. It could be used in the future research of Hypericum perforatum.