Objective: To investigate the inhibitory effect of radicamine B on glucose absorption in rat intestines i n vitro . Method: The inhibitory effect of radicamine B was evaluated by α-glucosidase inhibitory test

inhibitory kinetics assay and assay of glucose absorption in rat intestines in vitro . Result: Radicamine B showed an inhibitory effect on both α-glucosidase and glucose’s absorption in the small intestine in a dose-dependent manner and the IC50 value were 2.19 g·L-1 and 0.094 g·L-1

respectively. No significant difference was found between acarbose and radicamine B in the inhibitory effect on intestinal glucose absorption. In addition

the inhibitory pattern of radicamine B was competitive enzyme inhibition with its Ki of 6.3×10-7 mol·L-1. Conclusion: Radicamine B significantly inhibites glucose absorption in the small intestine. It indecates that radicamine B might be a novel therapeutic drug for diabetes.