Objective: To investigate the effect of Jiawei Fuzi Lizhong granule preparation on Bcl-2 and Bax gene expression in rats with aristolochic ACID nephropathy (AAN). Method: Ninety SD rats were divided into 5 groups randomly: normal control group (n=10)

model group (n=20)

Jiawei Fuzi Lizhong group (n=20)

prednisone group (n=20)

and Jiawei Fuzi Lizhong plus prednisone group (n=20). Experimental model of AAN was established by intragastric administration of aristoloch acid (100 mg·kg-1·d-1) for three days in SD rats. Rats in each group were intragastric administrated of distilled water (10 mL·kg-1·d-1)

distilled water (10 mL·kg-1·d-1)

Jiawei Fuzi Lizhong granule preparation (10 mL·kg-1·d-1)

prednisone (0.5 mg·kg-1·d-1)

and Jiaweifuzilizhong granule preparation (10 ml·kg-1·d-1) plus prednisone (0.5 mg·kg-1·d-1)

respectively. Three weeks later

Bcl-2 and Bax expression was examined. Renal tubular epithelial cells apoptosis was determined by TUNEL method. Result: Bcl-2 mRNA and protein expressions were obvious decreased

while Bax expressions increased. There was significant difference in Bcl-2 and Bax expressions between model group and the other experimental groups (P<0.05). Jiawei Fuzi Lizhong granule preparation plus prednisone group showed the strongest effect (P<0.05). The renal tubular epithelial cells apoptosis was obvious in model group. There were statistic differences in renal tubular epithelial cells apoptosis between model group and the other experimental groups (P<0.05). The Jiawei Fuzi Lizhong granule preparation plus prednisone group had the best effect on alleviating apoptosis (P<0.05). Conclusion: The Jiawei Fuzi Lizhong granule preparation had effect on reducing apoptosis rate in AAN rats. Furthermore

combination of Jiawei Fuzi Lizhong granule preparation and prednisone showed the best effect.