Objective: To study effects of the total flavones from Mori Folium(TFMF)on myocardial cell apoptosis and caspase-3 expression in myocardial ischemic-reperfusion injury in rats. Method: Ninety SD rats were randomized into sham operation group

model group

three dose groups of TFMF(35

70

140 mg·kg-1·d-1)

Tonxinluo capsule group(100 mg·kg-1·d-1).The left anterior descending coronary artery was ligated for 30 min

followed by 2 h of reperfusion to establish the rat model of myocardial ischemia-reperfusion injury. myocardia apoptosis were detected by the TUNEL staining and caspase-3 expression was detected by immunohistochemical technique method. Result: IS/LV and IS/AR increased obviously in model group compared with those in sham group

Myocardial apoptosis rate increased obviously(P<0.01)and caspase-3 protein expression was significantly elevated(P<0.05 or P<0.01)in the model group. Compared with model group

IS/LV

IS/AR and myocardial apoptosis rate in TFMF group decreased obviously(P<0.05 or P<0.01)

and caspase-3 protein expression was down-regulated(P<0.05 or P<0.01). Conclusion: TFMF possessed an obviouse ameliorate effect on myocardial cell apoptosis in myocardial ischemic-reperfused injury rats by decreasing the caspase-3 protein expression.