NKA-9型大孔树脂纯化蟾皮总生物碱工艺

王元清; 严建业; 罗堃; 王璐; 师白梅; 汪兰

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NKA-9型大孔树脂纯化蟾皮总生物碱工艺

更新时间：2024-01-04

- NKA-9型大孔树脂纯化蟾皮总生物碱工艺
- Purification Technology of Total Alkaloids from Skin of by NKA-9 Macroporous Resin
- 中国实验方剂学杂志 2011年17卷第22期页码：6-8
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- 基金信息：
  
  中南林业科技大学青年科学研究基金项目(2009015B);湖南省教育厅科学研究项目(11C1321);湖南省高校科技创新团队项目(湘教通[2010]212号)
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- 纸质出版日期：2011
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王元清, 严建业, 罗堃, 等. NKA-9型大孔树脂纯化蟾皮总生物碱工艺[J]. 中国实验方剂学杂志, 2011,17(22):6-8.

WANG Yuan-qing, YAN Jian-ye, LUO Kun, et al. Purification Technology of Total Alkaloids from Skin of by NKA-9 Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(22): 6-8.
王元清, 严建业, 罗堃, 等. NKA-9型大孔树脂纯化蟾皮总生物碱工艺[J]. 中国实验方剂学杂志, 2011,17(22):6-8. DOI：

WANG Yuan-qing, YAN Jian-ye, LUO Kun, et al. Purification Technology of Total Alkaloids from Skin of by NKA-9 Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(22): 6-8. DOI：

摘要

目的: 建立NKA-9型大孔树脂纯化蟾皮总生物碱的工艺条件。方法: 以蟾皮总生物碱含量(5-羟色胺计)作为考察指标

优化NKA-9型大孔树脂吸附与解析的工艺条件。结果: 优选出的最佳纯化工艺为NKA-9型大孔树脂在25 ℃进行吸附

样品溶液pH 9

上样量为4 BV

吸附时间为8~10 h

先用6 BV水洗

然后用3 BV70%的乙醇洗脱。结论: 该工艺可用于蟾皮总生物碱的纯化。

Abstract

Objective: To establish purification technology of total alkaloids from skin of Bufo bufo gargarizans by NKA-9 macroporous resin. Method: The content of total alkaloid (5-HT) from skin of B. bufo gargarizans was chosen as index

optimized condition of adsorption and desorption of NKA-9 macroporous resin and established purification technology condition of total alkaloids from skin of B. bufo gargarizans. Result: Optimum purification process conditions were:used NKA-9 macroporous resin

adsorption temperature was 25 ℃

loaded 4 times the amount of column volume with pH of sample solution was 9;adsorption time was 8-10 h

firstly eluted with 6 times water of column volume

then eluted with 3 times 70% ethanol of column volume. Conclusion: Optimum process could be used for purification of total alkaloids from skin of B. bufo gargarizans.

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