Objective: To study the antioxidant effect of sophoridine on lung injury induced by lipopolysaccharide(LPS)in mice and its influence on NF-κB expression. Method: Sixty Kunming mice were randomly divided into six groups: normal group

LPS-model group

prevention treatment group of sophoridine 5 mg·kg-1

three dose groups of sophoridine treatment (9

5

2.5 mg·kg-1)

ten mice in each group.Sophoridine was administrated for three days before LPS injection in prevention treatment group and administrated for three days after LPS injection in treatment groups.General condition of mice were observed before and after treatment.SOD and MDA in lung tissue homogenate was detected and pathological changes in lung tissue were observed.NF-κB p65 expression in lung tissue was determined by immunohistochemical method. Result: Compared with model group

general condition of mice was improved

and SOD in lung tissue homogenate increased(P<0.05)

MDA in lung tissue homogenate decreased(P<0.05)

pathological lesions of lung tissue alleviated in sophoridine treatment groups (5

2.5 mg·kg-1 dosage groups)and prevention treatment group.NF-κB p65 in lung tissue was reduced significantly in all sophoridine groups.And medium-dose sophoridine group showed the best effect. Conclusion: Sophoridine could reduce LPS-induced pathologic lesions in lung tissue of mice

protect from the damage caused by oxygen free radicals;sophoridine could reduce NF-κB expression

thus blocking LPS signal transduction pathway in TLR4-mediated NF-κB signal transduction pathway.