Objective: To develop an HPLC method for the determination of hyperin

hesperidin and resveratrol in Danning Tablets (DNT). Method: The chromatographic separation was performed on a Venusil XBP reversed phase C18 (4.6 mm×250 mm

5 μm) analytical column by gradient elution with acetonitrile and water buffer (containing 0.05% formic acid

adjusted with triethylamine to pH 5.0) at a flow rate of 0.8 mL ·min-1.The column temperature was at 25 ℃ and the optimum detection wavelength was set at 358 nm (hyperin)

283 nm (hesperidin) and 306 nm (resveratrol). Result: Three components were separated completely in 40 minutes. They had good linear relationship (r>0.999 6) and recovery (98.13%-101.19%) in their detection scope. Conclusion: The method is simple

accurate

and can be used for the quality control of this herbal medicine.