Objective: To optimize enzymatic hydrolysis technology of Gecko. Method: Folin phenol colorimetry was adopted to determine the content of small peptide

and used it as index

investigated dosage and reaction time of pepsin and trypsin respectively. Result: Optimum technology was as followed:added 5 times water

bioled

kept in room temperature

adjusted to pH 2.0 by diluted HCl

added 2.0% pepsin to medicinal quality

oscillated 2 h at 37 ℃

adjusted pH to 8.0

added 2.0% pepsin to medicinal quality

oscillated 4 h at 50 ℃

kept in room temperature

centrifuged(5 000 r·min-1×5 min)

separated supernatant

microfiltration(0.22 μm)

ultrafiltration(3 000)

collected filtration of relative molecular weight less than 3 000. Conclusion: Optimum enzymolysis process was steady.