Objective: To establish a high performance liquid chromatography method of simultaneously determining 6 constituents in Flos Lonicerae and Jinqi Jiangtang tablets. Method: The separation was carried out on an ODS C18 column (4.6 mm×250 mm

5 μm) with acetonitrile-0.1% H3PO4 as mobile phase by gradient elution. The flow rate was 1 mL·min-1 and the detection wavelength was at 355 nm. The column temperature was at 30 ℃. Result: The six ingredients were separated well. The linear ranges of chlorogenic acid

caffeic acid

rutin

hyperoside

luteolin-7-O-glucoside and 4

5-O-dicaffeoylquinic acid were 3.24-64.80

0.24-4.79

1.22-24.40

0.09-0.44

0.68-13.55

1.19-23.87 mg·L-1 respectively. The average recoveries were above 97% (RSD<3%

n=9). Conclusion: This method is simple and accurate with good recovery and repeatability

and can be used in Jinqi Jiangtang tablets and the related preparation quality control.