Objective: To observe the effect of Sheng-Yu decoction on peripheral blood cells

bone marrow cell cycle and apoptosis in myelosuppressed mice and to explore its possible mechanism in hematopoietic regulation. Method: The 60 BALB / c mice were randomly divided into blank group

model group

positive medicine group

Sheng-Yu decoction

three dose groups (32

16

8 g·kg-1·d-1)

with 10 mice in each group. Except the blank group. The other 5 groups were established the model of myelosuppression by irradiated 60Coγ and injection of cyclophosphamide and chloramphenicol. After 24 h of the completion of modeling

blank group and model group were ig normal saline

three doses of Sheng-Yu decoction groups were respectively ig corresponding crude drug 32

16

8 g· kg-1·d-1

with drug volume 20 mL·kg-1·d-1; and the positive control group were injected with rhG-CSF 125 ug· kg-1·d-1. Each group was medicated for 7 days.The effect of Sheng-Yu decoction on peripheral blood cells

bone marrow nucleated cells (BMNCs) and bone marrow cell cycle and apoptosis in myelosuppressed mice was detected by automatic blood cell analyzer

white blood cell counting and flow cytometry (FCM) respectively. Result: Peripheral white blood cells

red blood cells

hemoglobin

blood platelet and BMNCs were increased obviously by Sheng-Yu Granul

groups(P<0.05 or P<0.01). In model group

Sheng-Yu decoction (32

16 g·kg-1·d-1) dose groups

the proportion of G0/G1 phase cells were (70.38±1.38)%

(62.43±2.03)%

(63.85±2.90)%; S phase fraction was (22.52±1.27)%

(26.35±2.52)%

(24.80±1.48)%; G2 / M phase cells (7.43±1.04)%

(11.37±2.18)%

(10.95±2.35)%; PI were (29.85±1.06)%

(37.66±2.01)%

(35.88±3.13)%; apoptotic rates were (26.88±3.58)%

(19.15±4.30)%

(21.65±4.34)% respectively. Compared with the model group

the above parameters of Sheng-Yu Granul

groups were significantly different(P<0.05 or P<0.01). Conclusion: Sheng-Yu decoction could promote erythroid hematopoiesis by accelerating bone marrow cells of proliferation and diferentiation

inhibiting the apoptosis of hemopoietie cells.