HPLC同时测定莪术油中6种倍半萜成分

李文庭; 祝明; 陈碧莲; 唐登峰; 张鹏; 程勇

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HPLC同时测定莪术油中6种倍半萜成分

更新时间：2024-01-04

- HPLC同时测定莪术油中6种倍半萜成分
- Simultaneous Determination of Six Sesquiterpenes in Oleum Curcumae by HPLC
- 中国实验方剂学杂志 2011年17卷第20期页码：48-52
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家十一五支撑项目 (2009ZX09308-004);浙江省重大科技专项(2008C13037)
- DOI：
  中图分类号：
- 纸质出版日期：2011
- 稿件说明：
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李文庭, 祝明, 陈碧莲, 等. HPLC同时测定莪术油中6种倍半萜成分[J]. 中国实验方剂学杂志, 2011,17(20):48-52.

LI Wen-ting, ZHU Ming, CHEN Bi-lian, et al. Simultaneous Determination of Six Sesquiterpenes in Oleum Curcumae by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(20): 48-52.
李文庭, 祝明, 陈碧莲, 等. HPLC同时测定莪术油中6种倍半萜成分[J]. 中国实验方剂学杂志, 2011,17(20):48-52. DOI：

LI Wen-ting, ZHU Ming, CHEN Bi-lian, et al. Simultaneous Determination of Six Sesquiterpenes in Oleum Curcumae by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(20): 48-52. DOI：

摘要

目的: 建立反相高效液相色谱法同时测定莪术油中6种倍半萜类(莪术二酮、莪术醇、牻牛儿酮、呋喃二烯、莪术烯、β-榄香烯) 成分含量的方法。方法: 色谱条件 Agilent Extend C18色谱柱(4.6 mm×250 mm

5 μm)

流动相为乙腈(A)-甲醇(B)-水(C)

梯度洗脱(0~20 min

30%~65% A

40%~30% B;21~30 min

65% A

30% B)

流速1.0 mL·min-1

柱温30 ℃

检测波长216 nm。结果: 6种倍半萜类化合物莪术二酮、莪术醇、牻牛儿酮、莪术烯、呋喃二烯和β-榄香烯分别在为43.72~2 186

44.37~2 219

15.49~774.4

28.30~1 415

40.13~2 007

16.74~837.0 ng呈现良好的线性关系(r>0.999);平均加样回收率在98.46%~103.9%;6种倍半萜类化合物含量重复性试验RSD<1.29%。结论: 方法快速准确、具有良好的重复性和稳定性

可用于莪术油的质量控制。

Abstract

Objective: To develop an HPLC method for simultaneous determination of six sesquiterpenes (curdione

curcumol

germacrone

curzerene

furanodiene

β-elemene) in Oleum Curcumae. Method: HPLC was performed on an Agilent Extend Cl8 analytical column (4.6 mm × 250 mm

5 μm) with gradient elution (0-20 min

30%-65% A

40%-30% B

21-30 min

65% A

30% B)of acetonitrile (A) - methanol (B) - water (C) at the flow rate of 1.0 mL·min-1. The column temperature was maintained at 30 ℃. Detection wavelength was 216 nm. Result: The six sesquiterpenes had good linearity with correlation coefficient > 0.999

ranging from 43.72-2 186

44.37-2 219

15.49-774.4

28.30-1 415

40.13-2 007 ng and 16.74-837.0 ng respectively. The average recoveries were between 98.46%-103.9%

repeatability experiments showed that RSD values of their contents were less than 1.29%. Conclusion: The developed method is rapid

accurate with high repeatability and stability

which is helpful to control the quality of Oleum Curcumae.

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