HPLC-UV测定不同产地及不同采收期酸枣叶中芦丁的含量

裴香萍; 杜晨晖; 闫艳; 沈佳兴; 裴妙荣; 白瑶

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HPLC-UV测定不同产地及不同采收期酸枣叶中芦丁的含量

更新时间：2024-01-04

- HPLC-UV测定不同产地及不同采收期酸枣叶中芦丁的含量
- Determination of Rutin from Leaves of Gathered in Different Areas and Different Periods by HPLC-UV
- 中国实验方剂学杂志 2011年17卷第21期页码：94-96
- 作者机构：
- 作者简介：
- 基金信息：
  
  山西省卫生厅科技攻关青年项目(20100124)
- DOI：
  中图分类号：
- 纸质出版日期：2011
- 稿件说明：
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裴香萍, 杜晨晖, 闫艳, 等. HPLC-UV测定不同产地及不同采收期酸枣叶中芦丁的含量[J]. 中国实验方剂学杂志, 2011,17(21):94-96.

PEI Xiang-ping, DU Chen-hui, YAN Yan, et al. Determination of Rutin from Leaves of Gathered in Different Areas and Different Periods by HPLC-UV[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(21): 94-96.
裴香萍, 杜晨晖, 闫艳, 等. HPLC-UV测定不同产地及不同采收期酸枣叶中芦丁的含量[J]. 中国实验方剂学杂志, 2011,17(21):94-96. DOI：

PEI Xiang-ping, DU Chen-hui, YAN Yan, et al. Determination of Rutin from Leaves of Gathered in Different Areas and Different Periods by HPLC-UV[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(21): 94-96. DOI：

摘要

目的: 考察不同产地及不同采收期酸枣叶中芦丁的含量

以确定其最佳采收期

为酸枣叶的资源开发提供科学依据。方法: 采用HPLC

Diamonsil(钻石)C18色谱柱(4.6 mm×200 mm

5 μm )

流动相甲醇-0.1%冰醋酸(34 ∶66)

检测波长257 nm

流速1.0 mL·min-1

柱温30 ℃。结果: 芦丁在0.221 6~2.216 μg有良好的线性关系(r=0.999 9)

平均加样回收率为99.40% (RSD 2.42%

n=6);酸枣叶中芦丁含量一般以5~6月含量最高

7~8月芦丁含量有所下降

9~10月又有所升高。结论: 酸枣叶中芦丁的含量整体上随时间变化基本呈U字形趋势

该方法准确、简便、稳定

为酸枣叶的质量标准提供参考。

Abstract

Objective: To study the content of rutin from the leaves of Ziziphus jujuba gathered in different areas and different periods for ascertaining the best harvest time and providing rational basis for the resource development. Method: The assay was performed on a Diamonsil C18 (4.6 mm×200 mm

5 μm) column by an isocratic elution with methanol-0.1% acetic acid(34 ∶66) at a flow rate of 1.0 mL·min-1. The detection wavelength was at 257 nm.The column temperature was kept at 30 ℃. Result: Rutin was linear in the range of 0.221 6-2.216 μg (r=0.999 9). The average recovery ( n = 6) was 99.40% (RSD 2.42%). The content of rutin reached its maximum yields in May and June

however

the content drop down in July and August and rise again in September and October. Conclusion: This method was accurate

simple and stable. The content of rutin changing with time showed a U trend. It provided reference for quality evaluation criteria of the leaves of Z. jujuba

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