Objective: To establish an HPLC method for determination of ephedrine hydrochloride

D-pseudo-ephedrine and amygdalin in Xuanfei Zhike Oral Liquid. Method: The separation was performed on an Hedera ODS-2 (4.6 mm×250 mm

5 μm); the mobile phase consisted of acetonitrile-0.2%phosphoric acid (5:95) with the flow rate of 1 mL·min-1 for the determination of ephedrine hydrochloride

D-pseudo-ephedrine and 0.7 mL·min-1 for amygdalin

UV detection wavelength was at 210 nm and column temperature was 25 ℃. Result: ephedrine hydrochloride

D-pseudo-ephedrine and amygdalin were linear in the range of 0.043 2-0.684 μg (r=0.999 9)

0.048 8-0.976 μg (r=0.999 9) and 0.047 0-0.940 8 μg (r=0.999 8)

the average recoveries(n=6) of ephedrine hydrochloride

D-pseudo-ephedrine and amygdalin were 96.15%

96.09% and 95.74% with RSD of 0.78%

1.18% and 1.80% respectively. Conclusion: This method is rapid and accurate for the simultaneous analysis of ephedrine hydrochloride

D-pseudo-ephedrine and amygdalin and can provide a reliable way for the quality control for Xuanfei Zhike Oral Liquid.