Objective: To study the method of determining luteoloside in Reduping Granules. Method: The content of luteoloside in Reduping Granules was determined on an Thermo Hypeisil GOLD column (4.6 mm×250 mm

5 μm) eluted with acetonitrile and water containing 0.5% glacial acetic acid as mobile phases in gradient mode. The flow rate was kept at 1.0 mL·min-1

and the column temperature was set at 30 ℃. The UV detector wavelengths were at 350 nm. Result: Luteoloside was linear in the range of 0.034 7-0.694 μg(r=0.999 99). The average recovery was 99.3% and RSD was 0.5%. Conclusion: The established method is accurate and reliable

can be used to control the quality of this preparation effectively.