WEN Jin-lian, ZHOU Qing, SONG Fen-yun. Determination of Mangiferin in Zhizi Jinhua Wan by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(17): 65-67.
WEN Jin-lian, ZHOU Qing, SONG Fen-yun. Determination of Mangiferin in Zhizi Jinhua Wan by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(17): 65-67.DOI:
Objective: To establish the method for determination of mangiferin in Zhizi Jinhua Wan. Method: An RP-HPLC method was established. The chromatographic column was Diamonsil-C18. The mobile phase was a mixture of acetonitrile -0.2% phosphoric acid (13 ∶87). The flow rate was 1.0 mL ·min-1 and the detection wavelength was 258 nm. Result: The linear range of mangiferin was 5.1-30.6 mg ·L-1 (r=0.999 6). The average recovery for mangiferin was 99.0%
precision of the method was 1.7% (RSD
n=6). Conclusion: The method can be used for quantitative determination of the preparation.