Objective: To compare pharmacological activity and chemical composition changes of water extract from Huanglian Jiedu Decoction (HLJDT) after refined by 10 different Macroporous Resins. Investigated optimization principle of refining process by resin from traditional Chinese medicine compound

provided basis to refining and separation of traditional Chinese medicine compound prescription. Method: Ten different polarity type of polystyrene Macroporous Resins were selected to refine the water extract from HLJDT. Took activity tracking filter of rat adrenal medulla pheochromocytoma PC-12 cell

determined the effect of water extract and refined material to low-sugar of DMEM and the oxygen-glucose deprivation injury of cobalt chloride-induced PC-12cells by MTT. The content of six index ingredients (berberine

palmatine

baicalin

baicalein

wogonin and geniposide) were detected by RP-HPLC. Result: The results show:the refined material of water extract by X-5-type Macroporous Resin was better able to retain the existing ratio of each component in HLJDT

and the pharmacodynamic effects consistent with HLJDT. Conclusion: This study settled experimental basis to clinical improved formulations and of industrial production of HLJDT.