Objective: To investigate the feasibility of extracting the fat-soluble compounds from Magnolia officinalis with O/W microemulsion. Method: To measure the particle size of blank microemulsion by dynamic light scattering when it is being heated and compare with its physical index. To choose O/W microemulsion

water and ethanol as solvents to extract M. officinalis and determine the content of magnolol and honokiol in extracts of M. officinalis by HPLC. Compared with efficiency of extracting M. officinalis by using the different media

different formulations of O/W microemulsion and using different extraction methods and the differences of M. officinalis’ extracts by TLC. Result: The extracting yield of magnolol and honokiol are more than 90% by using microemulsion at mean time

which is similar to 60% ethanol. The formulations of microemulsion and extracting methods have remarkable effect for extracting the fat-soluble compounds from M. officinalis. There is no significant difference for blank microemulsion pre and post heated. Conclution: It is feasible to extract M. officinalis by using O/W microemulsion as a solvent. It could not only ensure the extracting efficiency of fat-soluble compounds

but also avoid the usage of organic solvents

save energy and reduce the links of manufacture

which are beneficial to the sustained development and low-carbon economy of Chinese Materia Medica.