Objective: To establish an HPLC method for determination of six major compounds in Dengzhanxixin injection

including chlorogenic acid

caffeic acid

1

3-O-dicaffeoylquinic acid

scutellarin

3

5-dicaffeoylquinic acid and 4

5-dicaffeoylquinic acid. Method: The analysis was carried on a Zorbax SB-C18 (4.6 mm×150 mm

5 μm) column. The mobile phase was composed of acetonitrile-0.1% formic acid with gradient elution. The flow rate was 1.0 mL·min-1. The detection wavelength was set at 327 nm. The column temperature was at 30 ℃

The injection volume was 20 μL. Result: The linear ranges of six compounds were as follows: 0.079-5.065 mg·L-1 (r=0.999 9) for chlorogenic acid

0.080-5.120 mg·L-1 (r=0.999 9) for caffeic acid

0.074-4.750 mg·L-1 (r=0.999 9) for 1

3-O-dicaffeoylquinic

0.391-25.050 mg·L-1 (r=0.999 9) for scutellarin

0.038-2.423 mg·L-1 (r=0.999 9) for 3

5-dicaffeoylquinic acid

and 0.487-7.792 mg·L-1 (r=0.999 8) for 4

5-dicaffeoylquinic acid. The average recoveries were 96.72% (RSD 1.76%)

96.64% (RSD 1.69%)

101.81% (RSD 1.81%)

98.16% (RSD 2.22%)

99.02% (RSD 2.57%)

97.02% (RSD 1.52%) respectively. Conclusion: The method is convenient

rapid and accurate with good reproducibility and could be used for the quality control of Dengzhanxixin injection.