Objective: According to the traditional Chinese medicine (TCM) theory that lung injury was resulted from chilled body shape and cold drink and"Yang "Deficiency was caused by over-strain and internal injury"

we combined two or three of four Lung-Yang Deficiency factors to compare the effects of these different combinations in. mice Lung-Yang Deficiency models. The four factors were termed as smoking in the special box

swimming in ice water

drinking ice water

and taking the methimazole ice solution. Method: Seventy ICR mice were randomly divided into four model groups and normal group. Each group with 14 animals was treated for 10 days. We observed the general physical signs (grip strength and self-activity)

measured MDA and SOD activity of lung tissue and serum

and calculated the organ indexes. Result: The body weight growth decreased

the self-activity and grip strength reduction

and the heart and lung indexes increase were observed in all model groups. In addition

the effects of three-factor combination groups on physical signs as well as MDA and SOD of lung tissue and serum were better than which of the two-factor combination groups

resulting in that swimming in ice water was more effective than drinking ice water. These results showed that the combination of smoking

swimming in ice water

and drinking ice water was the best method to induce Lung-Yang Deficiency. Conclusion: Different combinations of four Lung-Yang Deficiency factors indicated different effects. Among these combinations

the three-factor combination (smoking + swimming in ice water + drinking ice water) was cinsistent with TCM theory about Lung-Yang Deficiency

which may be induced by several factors such as chilled body shape

cold drinks and over-strain.