Objective: To establish HPLC fingerprint of Lvjiao Buxue granules for quality control of them. Method: An inertsil ODS2-C18 analytical column was used. The mobile phase A was 0.1% H3PO4 and mobile B was acetonitrile. The elution was in gradient mode and detection wavelength was set at 215 nm. The flow rate was 1.0 mL·min-1and the column temperature at 30 ℃.The analysis time was 95 min. Result: The similarity of 12 batches of Lvjiao Buxue granules was not lower than 0.90. Conclusion: This method is available for quality evaluation and can control the quality of Lvjiao Buxue granules.