乌药提取物对胃实寒模型大鼠cAMP, cGMP, GAS, MTL水平的影响

聂子文; 郭建生; 陈君; 张猛; 刘红艳

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乌药提取物对胃实寒模型大鼠cAMP, cGMP, GAS, MTL水平的影响

更新时间：2024-01-04

- 乌药提取物对胃实寒模型大鼠cAMP, cGMP, GAS, MTL水平的影响
- Effect of Extraction of Linderae Radix on cAMP cGMP GAS MTL Level in Rat Stomach Cold Models
- 中国实验方剂学杂志 2011年17卷第20期页码：162-165
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家科技部973资助项目(2007CB512603);国家重点基础研究发展计划项目(2007CB512600);湖南省科技计划项目(2007WK3033);湖南省中药重点学科
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  中图分类号：
- 纸质出版日期：2011
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聂子文, 郭建生, 陈君, 等. 乌药提取物对胃实寒模型大鼠cAMP, cGMP, GAS, MTL水平的影响[J]. 中国实验方剂学杂志, 2011,17(20):162-165.

NIE Zi-wen, GUO Jian-sheng, CHEN Jun, et al. Effect of Extraction of Linderae Radix on cAMP cGMP GAS MTL Level in Rat Stomach Cold Models[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(20): 162-165.
聂子文, 郭建生, 陈君, 等. 乌药提取物对胃实寒模型大鼠cAMP, cGMP, GAS, MTL水平的影响[J]. 中国实验方剂学杂志, 2011,17(20):162-165. DOI：

NIE Zi-wen, GUO Jian-sheng, CHEN Jun, et al. Effect of Extraction of Linderae Radix on cAMP cGMP GAS MTL Level in Rat Stomach Cold Models[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(20): 162-165. DOI：

摘要

目的: 观察乌药对胃实寒模型大鼠血浆中环磷酸腺苷(cAMP)、环磷酸鸟苷(cGMP)、胃泌素(GAS)、胃动素(MTL)的影响。方法: 模型组大鼠采用寒冷刺激法造模3 d制备胃实寒模型

按前后体重差随机分成8组

分别为模型对照组、吗丁啉组、红花水煎液组、乌药水煎液组、乌药挥发油组、乌药醇提液组、乌药水提乙醚萃取物组、乌药醇提乙醚萃取物组

乌药各提取物给药剂量均为生药1.64 g·kg-1

第4 d造模后10 min

给药组ig相应药液10 mL·kg-1

空白对照组及模型对照组给予等体积蒸馏水

1 h后进行第2次给药

以酚红法测定胃排空率。采用酶联免疫法测定大鼠血浆中cAMP

cGMP

MTL和血清GAS的含量。结果: 与正常组比较

模型组胃排空率显著降低(P<0.05)

cGMP

cAMP / cGMP降低(P<0.05)

cAMP

GAS

MTL值无显著差异。与模型组比较

乌药水煎液、乌药挥发油、乌药醇提乙醚萃取物能明显抑制胃排空率(P<0.05或P<0.01);乌药水煎液和乌药挥发油能明显升高cAMP含量(P<0.01)

乌药水煎液、乌药挥发油、乌药醇提液、能够明显降低cGMP含量(P<0.01)

乌药水煎液、乌药挥发油、乌药醇提乙醚萃取物能明显升高cAMP/cGMP(P<0.01)。结论: 乌药水煎液、挥发油和醇提乙醚萃取物组能够明显抑制胃排空

明显升高cAMP/cGMP。

Abstract

Objective: To observe the influence of Linderae Radix on cyclic adenosine monophosphate (cAMP)

cyclic guanosine monophosphate(cGMP)

gastrin (GAS)

motilin (MTL) of plasma in rat stomach cold models. Methods: The rats of model group were prepared by using cold stimulation method for three days. According to the difference of weight between before and after modelling

the rats were randomly divided into eight groups: model group

domperidone group

water decoction of Safflower group

the water decoction group

the volatile medicine group

the medicine ethanol-extraction liquid group

the potions mention ether extract group

the medicine ethanol-extraction ether extract group. At 10 minutes after modeling of the fourth day

treatment group was given corresponding solution 1 mL·kg-1

normal group and model group were given distilled water e

the second administration was carried out after one hour

the gastric emptying rate was identified through Phenol red method. the content of plasma cAMP

cGMP

MTL and serum GAS was examined. Result: Compared with the normal group

the gastric emptying rate of model group significantly reduced(P<0.05)

levels of cGMP

cAMP / cGMP were reduced(P<0.05)

and levels of CAMP

GAS

MTL had no significant difference.Compared with model group

treatment goups like the medicine decoction

volatile oil and uriah medicine ethanol-extraction ether extract of Linderae Radix significantly suppressed gastric emptying rate(P<0.05

P<0.01). The medicine decoction and volatile oil of Linderae Radix could obviously increase the content of cAMP(P<0.01); the medicine decoction

volatile oil

lindera root alcohol extract and alcohol extraction ether extract of Linderae Radix could obviously reduce the content of cGMP(P<0.01); the medicine decoction and volatile oil of Linderae Radix could significantly increased the ratio of cAMP/cGMP(P<0.01). Conclusion: The medicine decoction

volatile oil and uriah medicine ethanol-extraction ether extract of Radix Linderae can inhibit significantly gastric emptying

and can markedly elevate cAMP/cGMP.

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