LIU Ping-huai, WANG Chun-niu, CHEN De-li, et al. Determinnation of Antioxidant Capacity of accelerated solvent extraction (ASE) with DPPH Assay[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(21): 69-73.
LIU Ping-huai, WANG Chun-niu, CHEN De-li, et al. Determinnation of Antioxidant Capacity of accelerated solvent extraction (ASE) with DPPH Assay[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(21): 69-73.DOI:
Objective: To investigate the antioxidant effect of medicinal plants Vatica mangachapoi in Hainan province. Method: The sample extractions were prepared by accelerated solvent extraction (ASE) using solvent petroleum ether (MSO)
ethyl acetate (EtOAc)
ethanol (EtOH)
methanol (MeOH); Combining with microplate assay model
the ASEs that had high antioxidant activity were screened quickly. Result: The antioxidant activity of different solvent ASEs from Vatica mangachapoi was evaluated by DPPH assay. The antioxidant activity was listed as follows: ethyl acetate>ethanol>petroleum ether>methanol; its IC50 values were 0.143 4
0.501 8
0.567 7
0.693 7 g·L-1 respectively. The antioxidant of ethyl acetate ASE was better than ascorbic acid (IC50=0.203 4 g·L-1). Conclusion: The ethyl acetate ASE showed great DPPH scavenging activity
it’s hopeful to develop a new natural antioxidant.