Objective: To study the irritability of agglutinin from P. ternate Breit

P. pedtaisecta Schott and Typhonium giganteum Engl. Method: Male SD rats were designed to control group

low dose group(100 mg ·L-1)

medium dose group(200 mg ·L-1) and high dose group(300 mg ·L-1) randomly. The control group received the same volume of saline in substitution of the lectin solution. Four hours later

rats were sacrificed and peritoneal cells harvested by washing each peritoneal cavity with saline. The irritation effect of P. ternata agglutinin (PTA)

P. pedtaisecta agglutinin(PPA) and T. giganteum agglutinin(TGA) was observed in model of rat neutrophil migration in vivo by measurement of the number of neutrophils and the content of protein

NO and PGE2 of peritoneal fluid. Rabbits were designed to control group

Raphides of P. ternate group

P.pedtaisecta group and T. giganteum group

PTA and Raphide of P. ternate group

PPA and Raphide of P. pedtaisecta group

TGA and Raphide of T. giganteum group. Conjunctival congestion of rabbit was observed. Result: After injection of PTA

PPA and TGA

compared with control group (633.33 ± 233.81)×106/L the number of neutrophil migration in the low-dose group of PTA (1 083.33±116.9)×106/L

PPA (1 033.3±150.55)×106/L and TGA (1 133.33±103.28)×106/L was significant increased (P<0.01). Neutrophil migration was induced and the content of protein

NO and PGE2 was elevated by PTA

PPA

TGA. PTA

PPA

TGA that showed obvious irritation effects on conjunctival congestion of rabbit. Conclusion: Agglutinin is the irritative component from P. ternata

P. pedtaisecta and Typhonium giganteum.