竹节参总皂苷对脑缺血大鼠神经细胞凋亡和即早基因表达的影响

贾占红; 赵晖

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竹节参总皂苷对脑缺血大鼠神经细胞凋亡和即早基因表达的影响

更新时间：2024-01-04

- 竹节参总皂苷对脑缺血大鼠神经细胞凋亡和即早基因表达的影响
- Effects of TSPJ on Neuronal Apoptosis and Related Gene Expression in Rat Cerebral Ischemia-Reperfusion Injury
- 中国实验方剂学杂志 2011年17卷第21期页码：168-172
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- 基金信息：
  
  北京市优秀人材资助项目(20051D0501823);首都中医药与护理专项基金(11zyh07)
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- 纸质出版日期：2011
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贾占红, 赵晖. 竹节参总皂苷对脑缺血大鼠神经细胞凋亡和即早基因表达的影响[J]. 中国实验方剂学杂志, 2011,17(21):168-172.

JIA Zhan-hong, ZHAO Hui. Effects of TSPJ on Neuronal Apoptosis and Related Gene Expression in Rat Cerebral Ischemia-Reperfusion Injury[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(21): 168-172.
贾占红, 赵晖. 竹节参总皂苷对脑缺血大鼠神经细胞凋亡和即早基因表达的影响[J]. 中国实验方剂学杂志, 2011,17(21):168-172. DOI：

JIA Zhan-hong, ZHAO Hui. Effects of TSPJ on Neuronal Apoptosis and Related Gene Expression in Rat Cerebral Ischemia-Reperfusion Injury[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(21): 168-172. DOI：

摘要

目的: 观察竹节参总皂苷(TSPJ)对脑缺血再灌大鼠神经细胞凋亡和早期快速反应基因c-fos

c-jun表达的影响。方法: 60只SD大鼠随机分为假手术组、模型组、尼莫地平组、TSPJ组(200

100

50 mg·kg-1)。线栓法制备大鼠局灶性脑缺血-再灌注损伤模型;HE染色检测脑组织病理变化;TUNEL法检测神经细胞凋亡;免疫组化方法检测c-fos

c-jun的表达。结果: 脑缺血再灌注损伤后

模型组大鼠脑组织病理损伤明显

TUNEL细胞较假手术组显著增多(P<0.01)

c-fos

c-jun表达较假手术组明显增强(P<0.01);TSPJ可明显改善模型大鼠脑组织病理形态

TSPJ(200

100 mg·kg-1)组与模型组相比

TUNEL阳性细胞数明显减少(P<0.01或P<0.05)

c-fos

c-jun阳性表达减少(P<0.01或P<0.05)。结论: 对脑缺血损伤具有保护作用

其作用可能是通过下调c-fos

c-jun蛋白表达

从而干预脑缺血后的神经细胞凋亡。

Abstract

Objective: To investigate the effects of total saponins of Panax japonicus(TSPJ) on neuronal apoptosis and expression of c-fos

c-jun proteins in rat cerebral injury induced by ischemia and reperfusion. Method: Sixty SD rats were randomly divided into 6 groups:control group

model group

nimodipine group

TSPJ 200

100

50 mg·kg-1groups.The models of cerebral ischemia-reperfusion injury was reproduced by middle cerebral artery occlusion(MCAO). HE staining was used to assay the pathological changes;TUNEL stainning was used to detect apoptotic neurons;immuno-histochemistry was used to observe the protein expression of c-fos and c-jun. Result: Compared with the control group

the neuronal apoptosis was increased and the expression of c-fos、c-junhad remarkably enhanced after cerebral ischemia reperfusion(P<0.01);TSPJ improved the pathological changes

TSPJ(200

100 mg·kg-1)group significantly inhibited the expression of postitve neuronal cells of TUNEL and the expression of c-fos

c-jun decreased (P<0.01

P<0.05). Conclusion: TSPJ can decrease the number of apoptotic cells after cerebral ischemia and reperfusion by down-regulating the expression of c-fos and c-jun

which may be one of mechanisms of neuroprotective effects.

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