HPLC同时测定都梁滴丸中的阿魏酸、5-甲氧基补骨脂素、欧前胡素、异欧前胡素

席海为; 马强; 李强; 雷海民

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HPLC同时测定都梁滴丸中的阿魏酸、5-甲氧基补骨脂素、欧前胡素、异欧前胡素

更新时间：2024-01-04

- HPLC同时测定都梁滴丸中的阿魏酸、5-甲氧基补骨脂素、欧前胡素、异欧前胡素
- Simultaneous Determination of Ferulic Acid, 5-Methoxypsoralen, Imperatorin and Isoimperatorin in Duliang Dropping Pills by High Performance Liquid Chromatography
- 中国实验方剂学杂志 2011年17卷第16期页码：81-85
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- 纸质出版日期：2011
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席海为, 马强, 李强, 等. HPLC同时测定都梁滴丸中的阿魏酸、5-甲氧基补骨脂素、欧前胡素、异欧前胡素[J]. 中国实验方剂学杂志, 2011,17(16):81-85.

XI Hai-wei, MA Qiang, LI Qiang, et al. Simultaneous Determination of Ferulic Acid, 5-Methoxypsoralen, Imperatorin and Isoimperatorin in Duliang Dropping Pills by High Performance Liquid Chromatography[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(16): 81-85.
席海为, 马强, 李强, 等. HPLC同时测定都梁滴丸中的阿魏酸、5-甲氧基补骨脂素、欧前胡素、异欧前胡素[J]. 中国实验方剂学杂志, 2011,17(16):81-85. DOI：

XI Hai-wei, MA Qiang, LI Qiang, et al. Simultaneous Determination of Ferulic Acid, 5-Methoxypsoralen, Imperatorin and Isoimperatorin in Duliang Dropping Pills by High Performance Liquid Chromatography[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(16): 81-85. DOI：

摘要

目的: 建立一种同时测定都梁滴丸中的阿魏酸、5-甲氧基补骨脂素、欧前胡素、异欧前胡素含量的方法。方法: 都梁滴丸以75%甲醇超声提取30 min后取上清液

采用Agilent Zorbax SB C18(4.6 mm×150 mm

5 μm)色谱柱

以甲醇(A)-0.1%磷酸水溶液(B)为流动相进行梯度洗脱

流速1.0 mL·min-1;检测波长分别为254

320

330 nm。结果: 4种成分的方法最低定量限为3~15 mg·kg-1

线性范围0.5~50 mg·L-1

相关系数(r2)≥0.999 5。高、中、低3个添加水平的平均加样回收率(n=5)为96.6%~102.5%

RSD 0.16%~2.07%。结论: 该方法简便、快速适用于都梁滴丸中阿魏酸、5-甲氧基补骨脂素、欧前胡素和异欧前胡素的含量测定。

Abstract

Objective: To develop a high performance liquid chromatographic (HPLC) method for simultaneous determination of ferulic acid

5-methoxypsoralen

imperatorin

isoimperatorin in Duliang dropping pills. Method: Duliang dropping pills were extracted supersonically with 75% methanol for 30 min. The supernatant was separated on Agilent Zorbax SB C18 (4.6 mm×150 mm

5 μm) by gradient program with methanol (A)-water with 0.1% phosphoric acid (B) as the mobile phase at a flow rate of 1.0 mL·min-1

and determined by diode array detector at 254

320

330 nm respectively. Result: The limits of quantification of four compounds were in the range of 3-15 mg·kg-1

this method showed a good linearity between 0.5-50 mg·L-1 and the correlation coefficient (R2) was more than 0.999 0. The average recoveries were from 96.6% to 102.5% with relative standard deviation from 0.16% to 2.07%. Conclusion: This method is simple and rapid and suitable for determination of the four compounds in Duliang dropping pills.

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