Objective: To observe the effects of three factors with different combination on stability of vulnerable atherosclerotic plaque of carotid artery in rats. Method: With three factors

high lipid

2% L-methionine and carotid artery balloon injury

we established four models of vulnerable atherosclerotic plaque of carotid artery with their different combination

they were high lipid with carotid artery balloon injury

high lipid with 2%L-methionine

2%L-methionine with carotid artery balloon injury

and high lipid

2%L-methionine with carotid artery balloon injury(three factors model group). Each model group was given the relevant feed

and carotid artery balloon injury was operated at the fourth week. After 12 weeks

the levels of lipid

Hcy

hs-CRP and some plaque indexes were measured to evaluate four models. Result: In contrast to control group

the levels of TC

LDL-C

Hcy

and hs-CRP in three factors model group were raised significantly(P<0.01). In contrast to the others

the plague area related indexes(PA

PA/IEL-A

PA/EEM-A) of three factors model group were obvious. Conclusion: The atherosclerotic plaque of carotid artery in three factors group was obvious

the level of hs-CRP was raised

it showed that the plaque tended to rupture

and indicated there was synergistic effect among hyperlipemia

hyperhomocysteine (HHcy)

and vascular endothelial dysfunction. They worked together to make the formation and progress of the atherosclerotic vulnerable plaque of carotid artery.