HPLC测定知母-黄芪药对中芒果苷、 毛蕊异黄酮苷和毛蕊异黄酮

陈沛鑫; 李卫民; 高英; 帅颖

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HPLC测定知母-黄芪药对中芒果苷、毛蕊异黄酮苷和毛蕊异黄酮

更新时间：2024-01-04

- HPLC测定知母-黄芪药对中芒果苷、毛蕊异黄酮苷和毛蕊异黄酮
- Simultaneous Determination of Mangiferin, Calycosin-7--glucoside and Calycosin in Anemarrhenae Rhizoma-Astragali Radix by HPLC
- 中国实验方剂学杂志 2011年17卷第22期页码：72-75
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- 纸质出版日期：2011
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陈沛鑫, 李卫民, 高英, 等. HPLC测定知母-黄芪药对中芒果苷、毛蕊异黄酮苷和毛蕊异黄酮[J]. 中国实验方剂学杂志, 2011,17(22):72-75.

CHEN Pei-xin, LI Wei-min, GAO Ying, et al. Simultaneous Determination of Mangiferin, Calycosin-7--glucoside and Calycosin in Anemarrhenae Rhizoma-Astragali Radix by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(22): 72-75.
陈沛鑫, 李卫民, 高英, 等. HPLC测定知母-黄芪药对中芒果苷、毛蕊异黄酮苷和毛蕊异黄酮[J]. 中国实验方剂学杂志, 2011,17(22):72-75. DOI：

CHEN Pei-xin, LI Wei-min, GAO Ying, et al. Simultaneous Determination of Mangiferin, Calycosin-7--glucoside and Calycosin in Anemarrhenae Rhizoma-Astragali Radix by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(22): 72-75. DOI：

摘要

目的: 建立知母-黄芪中芒果苷、毛蕊异黄酮苷和毛蕊异黄酮3种有效成分的含量测定方法。方法: 采用HPLC

Phenomenex Luna C18(4.8 mm×250 mm

5 μm)色谱柱

加预柱(Security Guard

C18

3.0 mm×4.0 mm

Phenomenex);以乙腈-0.2%磷酸溶液为流动相

梯度洗脱

流速1.0 mL·min-1

柱温30 ℃

检测波长260 nm。结果: 芒果苷、毛蕊异黄酮苷和毛蕊异黄酮的线性范围分别为36.733~550.999 ng

33.376~166.88 ng

44.544~222.72 ng

Abstract

Objective: A reversed-phase high performance liquid chromatographic method (RP-HPLC) was developed for the simultaneous analysis of mangiferin

calycosin-7-O-β-D-glucoside and calycosin in Anemarrhenae Rhizoma-Astragali Radix. Method: HPLC determination was performed on a Phenomenex Luna C18 (4.8 mm×250 mm

5 μm) and detected at 250 nm. The mobile phase was consisted of acetonitrile and 0.2% phosphate solution with gradient elution. Flow-rate was 1.0 mL·min-1

and column temperature was 30 ℃. Result: The method was proved to be linear in the ranges of 36.733-550.999 ng

33.376-166.88 ng and 44.544-222.72 ng for mangiferin calycosin-7-O-β-D-glucoside and calycosin respectively. The average recoveries were 102.47%

102.38% and 100.20% respectively. Conclusion: The results showed that the method is convenient

accurate and reliable for the simultaneous determination of mangiferin

calycosin-7-O-β-D-glucoside and calycosin in Anemarrhenae Rhizoma-Astragali Radix.

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