微波协助提取在中药饮片含量测定中的应用(1)——微波法与药典法测定黄芩中黄芩苷含量比较研究

方婧; 付梅红; 杨洪军; 张贝贝; 王祝举; 杨岚; 张东

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微波协助提取在中药饮片含量测定中的应用(1)——微波法与药典法测定黄芩中黄芩苷含量比较研究

更新时间：2024-01-04

- 微波协助提取在中药饮片含量测定中的应用(1)——微波法与药典法测定黄芩中黄芩苷含量比较研究
- Determination of Microwave-assisted Extraction of Chinese Cut Crude Drugs (1) Comparison of the Determination of Baicalin from between Microwave-assisted Extraction and Chinese Pharmacopeia Method
- 中国实验方剂学杂志 2011年17卷第21期页码：46-48
- 作者机构：
- 作者简介：
- 基金信息：
  
  "重大新药创制"科技重大专项(2009ZX09308-003);国家科技支撑计划(2006BAI09B07-02)
- DOI：
  中图分类号：
- 纸质出版日期：2011
- 稿件说明：
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方婧, 付梅红, 杨洪军, 等. 微波协助提取在中药饮片含量测定中的应用(1)——微波法与药典法测定黄芩中黄芩苷含量比较研究[J]. 中国实验方剂学杂志, 2011,17(21):46-48.

FANG Jing, FU Mei-hong, YANG Hong-jun, et al. Determination of Microwave-assisted Extraction of Chinese Cut Crude Drugs (1) Comparison of the Determination of Baicalin from between Microwave-assisted Extraction and Chinese Pharmacopeia Method[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(21): 46-48.
方婧, 付梅红, 杨洪军, 等. 微波协助提取在中药饮片含量测定中的应用(1)——微波法与药典法测定黄芩中黄芩苷含量比较研究[J]. 中国实验方剂学杂志, 2011,17(21):46-48. DOI：

FANG Jing, FU Mei-hong, YANG Hong-jun, et al. Determination of Microwave-assisted Extraction of Chinese Cut Crude Drugs (1) Comparison of the Determination of Baicalin from between Microwave-assisted Extraction and Chinese Pharmacopeia Method[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(21): 46-48. DOI：

摘要

目的: 建立微波协助提取法提取黄芩中有效成分黄芩苷

比较微波协助提取法与药典法提取黄芩苷优势。方法: 采用Dikma Diamonsil C18色谱柱(4.6 mm×150 mm

5 μm)

流动相为甲醇-水-磷酸 (47 ∶53 ∶0.2)

检测波长280nm

柱温30 ℃

流速1 mL·min-1。结果: 微波提取时间15 min

提取温度120 ℃

提取溶剂30%乙醇。黄芩苷在0.059~0.59 μg呈良好线性关系

r=0.999 9。平均回收率99.36%( n =6)。结论: 微波提取法较药典法提取黄芩苷更简便快速、结果准确

为一种符合环保绿色理念的含量测定方法

可用于检测黄芩饮片中有效成分黄芩苷含量。

Abstract

Objective: To establish a microwave-assisted extraction(MAE)method for the extraction of the baicalin from Scutellaria baicalensis. Comparison of the determination of baicalin from S. baicalensis between MAE and Chinese pharmacopeia method. Method: Dikma Diamonsil C18 column(4.6 mm×150 mm

5 μm)was used. The mobile phase of methanol-water-phosphoric acid (47 ∶53 ∶0.2). The temperature of column was 30 ℃. The flow rate was 1.0 mL·min-1 and the detection wavelength was at 280 nm. Result: Microwave extractive time was 15 min

the temperature was 120 ℃

the extraction solvent was 60%ethanol.The calibration curve was in good linearity with in the range from 0.059 to 0.59 μg(r=0.999 9). The average recovery was 99.36% ( n =6). Conclusion: The method is simple

rapid

as well as precise and reliable

and can be used for the determination of baicalin in S. baicalensis.

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