Objective: To develop a HPLC method for determination of tanshinol

protocatechuic aldehyde

ferulic acid

salvianolic acid B

formononetin in compound preparation of Fuzheng Jiedu Keli. Method: The chromatographic separation was performed on a Dikmal ODS-C18 column (4.6 mm×250 mm

5 μm) maintaining at 30 ℃ during the whole process. The mobile phase consisted of methanol and 0.05%H3PO4 aqueous solution at a flow rate of 0.8 mL·min- 1. The detection wavelength was set at 280 nm for tanshinol

protocatechuic aldehyde

salvianolic acid B

316 nm for ferulic acid

248 nm for formononetin. Result: All the compounds were linear (r>0.999) in the range of the test concentrations

and the average recovery of the method was between 98% and 101%

RSD<3.55%. Conclusion: The method is proved to be credible

sensitive

accurate and repeatable. It can be applied to determine tanshinol

protocatechuic aldehyde

salvianolic acid B

ferulic acid

formononetin in compound preparation of Fuzheng Jiedu Keli simultaneously and provide a basal method of quality control to this preparation and other relative preparations.