Objective: To establish a plasma 8-acetylharpagide in HPLC determination of Khabarovsk to study different routes of administration in rats given 8-acetylharpagide Haba pharmacokinetics after. Method: Rats were injected and orally 8-acetylharpagide

blood was collected at different time points; detected in plasma by HPLC 8-acetylharpagide

acetonitrile-isopropyl alcohol-water (1.8 ∶10.2 ∶88)

detection wavelength 207 nm

flow rate 1.0 mL· min-1

draw the curve

the establishment of pharmacokinetic models

calculation of pharmacokinetic parameters. Result: Regression equation of 8-acetylharpagide in plasma was Y=613 753.13X －86 995.55(r=0.999 2).In the range of 0.015 3-22.995 μg with good linear relationship.The intra-day and inter-day precision of three dosage were 0.59%

0.38%

0.55%;3.9%

2.1%

1.5% respectively.The extraction recoveries were 94.89%

95.85%

95.25%.The intragastric approach basically can not be detected in thebody;The main pharmacokinetic parameters of injection means by three dosage group were as follows:t1/2(21.15±3.36)

(24.26±5.50)

(16.58±5.65) min;tmax (4.98±0)

(4.98±0)

(4.98±0) min;Cmax (2.25±1.41)

(0.09±0.03)

(0.02±0.007) g·L-1. Conclusion: This method was proved to be sensitive

selective and simple.It can be successfully applied to the pharmacokinetic study of 8-acetylharpagide.The injection given to 8-acetylharpagide in rats in vivoabsorption of Khabarovsk fast and does not completely eliminate relatively slow

but with good pharmacokinetic characteristics

compartment model fitting analysis for the compartment model.