Objective: To investigate the pathological change of H22 hepatoma induced by radioactive particle125I combine with cisplatin(DDP). Method: 125I

Cisplatin (DDP) and 125I combined with DDP were implanted into the mice H22 hepatoma

individually.After 28 days

the tumor growth was observed

and the pathological change in the cells were investigated by optical microscope and electron microscope. Result: After125I

DDP were implanted into the mice H22 hepatoma

the tumor growth showed fast at first phase and then became slow;125I combined with DDP made the tumor growth slower with cell degeneration and necrosis.Under the optical microscope

cells of125I group and DDP group had a irregular shape

part of cells showed necrosis and surrounding tissue cells had lots of lymphocytes.But the characteristics of125I combined with DDP was cells necrosis.Under the electron microscope

the nuclear membrane was complete

chromatin distributed uniform

nucleolus was concentrated or fragmentated and the apoptotic bodies were form.The configuration of rough endoplasmic reticulum was loosening and degranulated

with increasing endoplasmic reticulum

which had more apoptosis of cancer cells

and had more apoptotic cells aggregated in the125I group and DDP group.But the125I combined with DDP group was with necrosis or incomplete necrotic cells mainly

which also had the apoptosis cancer cells. Conclusion: The interstitial implant of radioactive seed125I induces hepatoma cells apoptosis. 125I combined with DDP induces apoptosis

cell degeneration and necrosis.