Objective: To observe the protective effect of the compound Ginkgo biloba (CGB)

the combination of Ginkgo biloba extracts (GBE) and Rose roxburghii

on hepatic injury and to explore the possible mechanism. Method: Eighty-four male rats of clean grade were randomly divided into 7 groups: control group

acute liver injury model group

GBE group(0.4 g ·kg-1)

and low

middle and high doses of CGB groups(0.4

0.8

2.4 g ·kg-1)

bifendatatum group. These rats were firstly given with corresponding drugs for 4 weeks

and then were induced by alcohol at a dose of 6 g ·kg-1 except for the control group

and after 6 hour the rats were sacrificed

and ALT

AST in serum and MDA

TG in tissue were assayed. The in vitro damage of L02 cells by hydrogen peroxide (H2O2) was chosen to establish the model of hepatocellular oxidative stress and the levels of ALT

AST and TAOC were determined. Result: Compared with model group

the levels of AST

ALT and TG in groups of CGB high and medium doses were decreased significantly (P<0.05 or P<0.01)

and the levels of MDA in groups of CGB high and low doses were decreased significantly (P<0.05). Except the levels of AST in the low doses of CGB

the exaltation of ALT and AST of LO2 cells stimulated in vitro by H2O2 were inhibited (P<0.05 or P<0.01) and the capacity of TAOC was enhanced in the groups of CGB and GBE (P<0.01). The effects of high dose and medium dose groups of CGB were better than that of GBE for decreasing the levels of AST

ALT and increasing the capacity of TAOC. Conclusion: CGB can protect the hepatocyte injury induced by alcohol and H2O2.It might be related to its collaborative antioxidation.