HPLC同时测定痛宁凝胶中延胡索乙素和原阿片碱的含量

王伟; 尚强; 杨璐; 毕宇安

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HPLC同时测定痛宁凝胶中延胡索乙素和原阿片碱的含量

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- HPLC同时测定痛宁凝胶中延胡索乙素和原阿片碱的含量
- HPLC Determination of Protopine and Tetrahydropalmatine in Tongning Gel
- 中国实验方剂学杂志 2011年17卷第12期页码：93-95
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家科技部重大新药创制项目(2009ZX09313-032)
- DOI：
  中图分类号：
- 纸质出版日期：2011
- 稿件说明：
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王伟, 尚强, 杨璐, 等. HPLC同时测定痛宁凝胶中延胡索乙素和原阿片碱的含量[J]. 中国实验方剂学杂志, 2011,17(12):93-95.

Wang Wei, SHANG Qiang, YANG Lu, et al. HPLC Determination of Protopine and Tetrahydropalmatine in Tongning Gel[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(12): 93-95.
王伟, 尚强, 杨璐, 等. HPLC同时测定痛宁凝胶中延胡索乙素和原阿片碱的含量[J]. 中国实验方剂学杂志, 2011,17(12):93-95. DOI：

Wang Wei, SHANG Qiang, YANG Lu, et al. HPLC Determination of Protopine and Tetrahydropalmatine in Tongning Gel[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(12): 93-95. DOI：

摘要

目的: 建立痛宁凝胶中延胡索乙素和原阿片碱的含量测定方法。方法: 采用高效液相色谱法

色谱柱为Kromasil C18柱(4.6 mm × 250 mm

5 μm)

以乙腈-磷酸盐溶液为流动相进行梯度洗脱

检测波长280 nm

柱温30 ℃

流速1.0 mL · min-1。结果: 原阿片碱在0.100 6 ~5.03 μg呈良好的线性关系(r=1.0)

平均回收率为99.14%

RSD 1.34%(n=6);延胡索乙素在0.100 8 ~5.04 μg呈良好的线性关系

r =1.0

平均回收率为101.1%

RSD 1.18%(n=6)。结论: 方法操作简便、可靠

重现性好

专属性强

能有效控制该制剂的质量。

Abstract

Objective: To establish an HPLC method for determination of protopine and tetrahydropalmatine in Tongning Gel. Method: The liquid chromatography was carried out using Kromasil C18 (4.6 mm × 250 mm

5 μm)

with detection wave length at 280 nm

acetonitile-phosphate buffer as mobile phase

gradient elution.The column temperature was at 30 ℃ and the flow rate was 1.0 mL · min-1. Result: The calibration curve of protopine was linear in the range of 0.100 6-5.03 μg(r=1)

and the recovery was 99.14% (RSD 1.34%). The calibration curve of tetrahydropalmatine was linear in the range of 0.100 8-5.0 4 μg(r=1)

and the recovery was 101.1%(RSD 1.18%). Conclusion: This method is simple

accurate

reliable and reproducible

which can be used for quality control of Tongning Gel.

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