Objective: To study the chemical components and antimicrobial activity of the essential oil from the flower of Senecio scandens. Method: The chemical components of the volatile oil derived from flower of S. scandens were extracted by simultaneous distillation-extraction and analyzed by GC-MS. The antimicrobial activity of the essential oil was tested by cylinder plate method. Result: Twenty-eight compounds of essential oil were identified and accounting for 89.59% of the total volatile oil. The main constituents in the essential components were (E) ocimene (14.60%)

nerolidol (13.57%)

α-pinene (12.95%)

(Z) ocimene (11.67%)

2-naphthylamine (6.98%). The oil was found to inhibit the growth of Bacillus subtilis

but not of Escherichia coli. and Staphylococus altrells. Conclusion: Analysis can provide evidence for development and usage of flower of S. scandens.