反相高效液相色谱法测定新疆枸杞叶茶中 芦丁和槲皮素的含量

刘波; 张浩科; 宫海燕

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反相高效液相色谱法测定新疆枸杞叶茶中芦丁和槲皮素的含量

更新时间：2024-01-04

- 反相高效液相色谱法测定新疆枸杞叶茶中芦丁和槲皮素的含量
- Determination of Rutin and Quercetin in Tea of Leaves by RP-HPLC
- 中国实验方剂学杂志 2011年17卷第14期页码：92-94
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- 纸质出版日期：2011
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刘波, 张浩科, 宫海燕. 反相高效液相色谱法测定新疆枸杞叶茶中芦丁和槲皮素的含量[J]. 中国实验方剂学杂志, 2011,17(14):92-94.

LIU Bo, ZHANG Hao-ke, GONG Hai-yan. Determination of Rutin and Quercetin in Tea of Leaves by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(14): 92-94.
刘波, 张浩科, 宫海燕. 反相高效液相色谱法测定新疆枸杞叶茶中芦丁和槲皮素的含量[J]. 中国实验方剂学杂志, 2011,17(14):92-94. DOI：

LIU Bo, ZHANG Hao-ke, GONG Hai-yan. Determination of Rutin and Quercetin in Tea of Leaves by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(14): 92-94. DOI：

摘要

目的: 采用反相高效液相法测定新疆枸杞叶茶中的芦丁和槲皮素的含量。方法: Kromasil C18色谱柱(4.6 mm ×250 mm

5 μm)

流动相甲醇-0.04%磷酸水溶液(用三乙胺调pH 3.0)(45 ∶55)

检测波长360 nm

流速1.0 mL ·min-1。结果: 芦丁和槲皮素的线性范围分别在0.23~1.84 μg和0.03~0.24 μg

回收率分别为98.19%

97.98%。RSD分别为1.49%

2.25%。结论: 本方法简便、可靠、重现性较好

为新疆枸杞叶茶的质量控制提供了快速、准确的测定方法。

Abstract

Objective: To determine active constituents quercetin and rutin in tea of Lycium barbarum leaves by RP-HPLC. Method: Rutin and quercertin were separated on an Kromasil C18 column (4.6 mm×250 mm

5 μm) with methanol-0.04% phosphoric acid (pH was adjusted to 3 with diethylamide) (45 ∶55) as mobile phases. The detecting wavelength was at 360 nm. The flow rate was 1.0 mL ·min-1. Result: The linear ranges of rutin and quercertin were within 0.23-1.84 μg and 0.03-0.24 μg respectively. The recoveries were 98.19% and 97.98%

RSDs were 1.49% and 2.25% respectively. Conclusion: The method is simple

accurate

and reliable

which provides the basis of a better development for the tea of L. barbarum in Xinjiang.

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