Objective: To study the chemical constituents from Momordica charantia. Method: M. charantia was extracted by alcohol and purified by macroporous adsorptive resin. The isolation and purification were carried out by silica gel and semi-prepared HPLC

the compounds were identified and elucidated by spectral and chemical methods. Result: Six compounds were obtained and identified as charantadiol A(Ⅰ)

soya-cereboiside Ⅰ(Ⅱ)

Naringin(Ⅲ)

(3β

20R

23R)-3-{O-6-deoxy-α-L-mannopyranosyl-(1-2)-O- -6-O-acetyl-β-D-glucopyranosyl oxy}-20

23-dihydroxydammar-24-en-21-oic acid 21

23-lactone(Ⅳ)

β-sitosterol (Ⅴ) and daucosterol (Ⅵ). Conclusion: Compound Ⅲ and Compound Ⅳ are obtained from M. charantia for the first time. Compound Ⅳ also firstly isolated from this genus.