WAN Zhi-qiang, YAN Ming-ming, ZHAN Yue, et al. Separation,Purification and Determination of Protein in [J]. Chinese journal of experimental traditional medical formulae, 2011, 17(17): 116-118.
WAN Zhi-qiang, YAN Ming-ming, ZHAN Yue, et al. Separation,Purification and Determination of Protein in [J]. Chinese journal of experimental traditional medical formulae, 2011, 17(17): 116-118.DOI:
Objective: To develop a simple method for effectively extracting protein from schisandra with the comparison of three extractive methods. Method: The extracts of the three methods were determined with SDS-PAGE
the content of protein was determined with the Lowry method
and a HPLC method was established for the determination of the amino acids in schisandra extract. Result: The protein content of the three methods was 58.44%
19.21% and 26.18%.The amino acids content of the three methods was 57.46%
15.41% and 23.14%. Conclusion: Purified protein and its amino acids were obtained by defatted ammonium sulfate precipitation procedure
and the content of protein was acceptable.This method is easily and more feasible.