Objective: Yupingfeng San (YPFS) is a traditional formula used for allergy such as asthma

allergic rhinitis

and urticaria. Mice Th2 cell-mediated allergic contact dermatitis was utilized in this study to explore the effect of YPFS on allergic inflammatory. Method : The mice were topically sensitized on the shaved abdomens with 1.5%FITC solution on abdominal skin in the day 1 and day 2 and elicited on the right ear with 0.5%FITC solution at day 6. Mice were treated with either YPFS or normal saline from day 1 to day 5 (induction phase). Ear thickness was measured using a calibrated thickness gauge 24 h after the elicitation. Ear pathohistological examination was carried out by HE staining and IL-4 level of ear tissue was detected by ELISA. Result : The ear thickness

measured either by calibrated thickness gauge or under the microscope

was inhibited significantly by administration with YPFS 6.5 g ·kg-1 and YPFS 13 g ·kg-1 in induction phase. The pathohistological examination showed that the ear inflammation and infiltration of inflammatory cells was significantly reduced by YPFS. The levels of IL-4 in ear tissue were significantly lower in YPFS 6.5 g ·kg-1 group compared with that in model group. Conclusion : Administration with YPFS in induction phase could inhibit Th2 cell-mediated allergic contact dermatitis in mice significantly

and the mechanism may be related with regulating IL-4.