响应面法优化贯叶连翘中金丝桃素超声提取工艺

郭凯; 梁剑平; 华兰英; 王学红; 石广亮; 刘宇

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响应面法优化贯叶连翘中金丝桃素超声提取工艺

更新时间：2024-01-04

- 响应面法优化贯叶连翘中金丝桃素超声提取工艺
- Optimization of ultrasonic extraction of hypericin in Hypericum perforatum L. using response surface methodology
- 中国实验方剂学杂志 2011年17卷第11期页码：7-12
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- 基金信息：
  
  “十一五”国家科技支撑计划
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- 纸质出版日期：2011
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郭凯, 梁剑平, 华兰英, 等. 响应面法优化贯叶连翘中金丝桃素超声提取工艺[J]. 中国实验方剂学杂志, 2011,17(11):7-12.

GUO Kai, liangjianping, hualanying, et al. Optimization of ultrasonic extraction of hypericin in Hypericum perforatum L. using response surface methodology[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(11): 7-12.
郭凯, 梁剑平, 华兰英, 等. 响应面法优化贯叶连翘中金丝桃素超声提取工艺[J]. 中国实验方剂学杂志, 2011,17(11):7-12. DOI：

GUO Kai, liangjianping, hualanying, et al. Optimization of ultrasonic extraction of hypericin in Hypericum perforatum L. using response surface methodology[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(11): 7-12. DOI：

摘要

【目的】本文以贯叶连翘为原料，应用响应面法探求超声提取金丝桃素的最优工艺。【方法】在对影响金丝桃素提取率的单因素系统考察的基础上，采用部分析因设计分析筛选以上因素，并应用响应面法对其显著因素进行研究和优化。【结果】建立了超声提取贯叶连翘中金丝桃素的工艺数学模型。得到了超声提取金丝桃素的最佳工艺条件：超声提取时间为36.15 min、丙酮/乙醇混合提取溶剂中丙酮体积分数为68.14%、液料比为11.87 mL?g-1，此时金丝桃素提取率理论值可达到1.454%。经验证，提取率为1.44%，与理论值相符。得到主效应关系为：超声提取时间＞丙酮体积分数＞液料比。【结论】通过以上实验得到的提取工艺条件可靠，金丝桃素提取率远高于常规提取方法，可为金丝桃素的工业化放大生产提供了一定的理论依据。

Abstract

The objective of the current study was to determine the optimum ultrasonic extraction conditions for hypericin from Hypericum perforatum L. The optimum conditions were evaluated with 2-level fractional factorial design and optimized using response surface methodology. High yields of hypericin were observed from Hypericum perforatum L. by ultrasonic extraction technology. The extraction time

acetone concentration and the ratio of solvent to material were the most significant parameters on hypericin extraction as evaluated through FFD. The extraction conditions were further investigated with central composite design. The results showed that the optimal parameters for this method were: extraction time of 36.15 min

acetone concentration of 68.14(Vol) of acetone/ethanol solvent

and ratio of solvent to material of 11.87 mL?g-1. Under the optimized condition

the maximum productivity of hypericin is 1.44%. The yield of hypericin under the optimized extraction conditions were great higher than that obtained from conventional solvent extraction. The present study demonstrates that ultrasonic is a great efficient tool for the fast extraction of hypericin from Hypericum perforatum L.

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