Objective: To observe the effect of Shugan Jianpi Recipe on 5-hydroxytryptamine(5-HT)

calcitonin gene related peptide(CGRP) in rats with diarrhea predominant irritable bowel syndrome(D-IBS). Method: Sixty Wistar rats were randomized into normal group

model group

low

middle and high-dose groups of Shugan Jianpi Recipe

and dicetel group.Cold-binding applications were given to establish similar model of D-IBS.Violet spectrophotometer was used for determining the levels of 5-HT in serum.RIA was used for measuring the levels of CGRP in serum.The expressions of 5-HT and CGRP in intestinal and colon mucosa and colon tissue were detected by immunohistochemical SABC method. Result: In the model group

the 5-HT level increased

CGRP decreased

compared with those in the normal group(P<0.05).The Shugan Jianpi Recipe's middle

high-dose increased 5-HT and decreased CGRP significantly

compared with that in the model group(P<0.01). Conclusion: The therapeutic mechanism of Shugan Jianpi Recipe for D-IBS is probably related with adjustment of 5-HT and CGRP in serum and colon tissue.