Objective: The content of aristolochic acid A in the five kinds of medical materials and dispensing granules of Aristolochiaceae family was determined to provide scientific foundation for promoting development of our traditional Chinese medicine particle. Method : HPLC method was applied on YMC-Pack C18 column (4.6 mm×250 mm

5μm) at 30 ℃ with methanol (A)-0.05% phosphoric acid (B) as the mobile phase; detected wavelength was at 250 nm

and the flow rate was 0.8 mL ·min-1. Result : The content of aristolochic acid A in the five kinds of medical materials

i.e. Akebia stem

Dutchmanspipe Fruit

honeyed Dutchmanspipe Fruit

Radix Aristolochiae and Datchmanspipe Herb was 4.18 mg ·g-1

0.77 mg ·g-1

0.46 mg ·g-1

0.86 mg ·g-1

0.53 mg ·g-1 respectively. The content of aristolochic acid A in the five kinds of dispensing granules was 1.16 mg ·g-1 for Akebia stem

0.31 mg ·g-1 for Dutchmanspipe Fruit

0.10 mg ·g-1 for honeyed Dutchmanspipe Fruit

0.39 mg ·g-1 for Radix Aristolochiae and 0.03 mg ·g-1 for Datchmanspipe Herb respectively. Conclusion : The method established in this paper has relative adaptability for various kinds of medical materials and dispensing granules containing aristolochic acid A. It can be used for content determination. The production process of traditional Chinese medicine particle prescription has certain effect to composition of medical materials

so it is necessary to establish relevant production monitoring criterion

quality monitoring criterion for ensuring the quality of traditional Chinese medicine particle prescription.